Literature references

References pertaining to infraluciferin:

Willey TL, Squirrell DJ and White PJ (2001). Design and selection of firefly luciferases with novel in vivo and in vitro properties. In Proc. 11th Int. Symp. Biolum. Chemilum. Editors: Case JF, Herring PJ, Robinson BH, Haddock SHD, Kricka LJ and Stanley PE. World Scientific, Singapore. Pgs 201-4.

Law GHE, Gandelman OA, Tisi LC, Lowe CR and Murray JAH (2006). Mutagenesis of solvent exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance. Biochem. J. 397: 305-312.

Jathoul A, Law E, Gandelman O, Pule M, Tisi L and Murray J (2012a). Development of a pH tolerant thermostable Photinus pyralis luciferase for brighter in vivo imaging. In: Lapota D, editor. Bioluminescence - recent advances in oceanic measurements and laboratory applications. London: IntechOpen.

Jathoul AP, Philip B, Thomas S and Pule MA (2012b). Characterisation of a bright and thermostable rainbow of firefly luciferases: enhanced tools for in vivo bioluminescence imaging. World Molecular Imaging Conference poster session, 6th September 2012, Dublin, Ireland, UK. (( Nb. In this poster x11 green (x11 + V241I, G246A, F250S) and x11 red2 (x11 + S284T, R354I), or simply called x11 red in this poster, were first engineered and are called ‘FLuc_green’ and ‘FLuc_red’ in Stowe et al., 2019 (Elife 8: e45801). x11 red2 had proven very effective for NIR BLI with infraluciferin when tested in mouse models at UCL during 2013-2014 (unpublished).

Patent for infraluciferin held by Bioflares Ltd: Derivatives of luciferin (2014). EP 2981527 A1 (text from WO2014162157A1). Inventors: Amit P. JATHOUL, Helen GROUNDS, James C. Anderson, Martin A. PULE.

Jathoul AP, Grounds H, Anderson JC, Pule MA (2014). A dual-color far-red to near-infrared firefly luciferin analogue designed for multiparametric bioluminescence imaging. Angew. Chem. Int. Ed. Engl. 53: 13059-63.

Berraud-Pache R and Navizet I (2016). QM/MM calculations on a newly synthesised oxyluciferin substrate: new insights into the conformational effect. Phys. Chem. Chem. Phys. 18: 27460-7.

Anderson JC, Grounds H, Jathoul AP, Murray JAH, Pacman SJ and Tisi LC (2017). Convergent synthesis and optical properties of near-infrared emitting bioluminescent infra-luciferins. RSC Adv. 7: 3975-82.

Woodhouse JL, Assmann M, Parkes MA, Grounds H, Pacman SJ, Anderson JC, Worth GA and  Helen H. Fielding HH (2017). Photoelectron spectroscopy of isolated luciferin and infraluciferin anions in vacuo: competing photodetachment, photofragmentation and internal conversion. Phys. Chem. Chem. Phys. 19: 22711-20.

Halliwell LM, Jathoul AP, Bate JP, Worthy HL, Anderson JC, Jones DD and Murray JAH (2018).ΔFlucs: Brighter Photinus pyralis firefly luciferases identified by surveying consecutive single amino acid deletion mutations in a thermostable variant. Biotechnol. Bioeng. 115: 50-9.

Abdulla M, Jathoul A, Vinh N, et al (2018). I23 Tracking transplanted human foetal cells using bioluminescence imaging in a huntington’s disease model. Journal of Neurology, Neurosurgery & Psychiatry 2018;89:A96-A97.

Anderson JC, Chang CH, Jathoul AP and Syed AJ (2019). Synthesis and bioluminescence of electronically modified and rotationally restricted color-shifting infraluciferin analogues. Tetrahedron 75: 347-56.

Stowe C, Burley TA, Allan H, Vinci M, Kramer-Marek G, Ciobota DM, Parkinson GN, Southworth TL, Agliardi G, Hotblack A, Lythgoe MF, Branchini BR, Kalber TL, Anderson JC and Pule MA (2019). Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin. Elife 8: e45801.

Jathoul AP, Branchini BR, Anderson JC and Murray JAH (2022). A higher spectral range of beetle bioluminescence with infraluciferin. Front. Bioeng. Biotechnol. Sec. Biomaterials. Online:

Chang CH, Gómez S, Fontaine DM, Fikas P, Branchini BR  and Anderson JC (2023). Bioluminescence, photophysical, computational and molecular docking studies of fully conformationally restricted enamine infraluciferin. Org. Biomol. Chem. 21: 2941-2949.