Dual near-infrared (NIR) bioluminescence with infraluciferin can be used for tracking and quantifying both Chimeric Antigen Receptor (CAR) T-cells and their cancer targets at the same time, in a single mouse:
Advantages of infraluciferin:
1. The killing of cancer by the T-cells can be assessed in living animals and this can help enable faster preclinical testing of CAR T-cell therapies (receptors) in different mouse models (Stowe et al., 2019): image two biological parameters at once
2. This ability is coupled to the advantages of far red to NIR imaging: more accurate in vivo imaging (better quantification & image rendering)
3. This could halve the amount of animals used for CAR imaging
4. It's also a perfect way to test your CAR T-cell safety (suicide) switch in vivo
The Bioflares schematic below shows the use of infraluciferin & ilucs for effector and target dual NIR BLI in mice: see your CAR T-cell experiments in living animals:
For selected literature references on infraluciferin click here